Table 2. Effect of some inhibitors on tryptic activity (using BApNA as substrate) of salivary gland extract.
| Inhibitor | Target proteases | % inhibition |
|---|---|---|
| PMSF(1 mM) | Serine proteases | No inhibition |
| E-64 (1 μM) | Cysteine proteases | No inhibition |
| E-64 (10 μM) | Cysteine proteases | 5.4±2.04 |
| Leupeptin (10 μg/ml) | Serine and cysteine proteases | 73.35±2.01 |
| TLCK(0.1 mM) | Trypsin-like serine proteases | 78.37±1.02 |
| SKTI (10 μg/ml) | Trypsin-like serine proteases | No inhibition |
| TPCK(0.1 mM) | Chymotrypsin-like serine proteases | 5.98±2.22 |
| Chymostatin (100 μM) | Chymotrypsin-like serine and cysteine proteases | 24.17±0.77 |