Figure 2. Water loss as a function of temperature in female adults of Mezium affine on a linear plot before (A, living; B, killed) and after cuticular lipid extraction (C, delipidized); D, same data re-plotted on an Arrhenius plot to determine activation energy, E_a (slope of regression = −E_a/R_gas). HCN-killed beetles produced nearly identical results and results were similar for ramp up and ramp down experiments. n = 30 beetles for each individual point on the graph (± SE ≤ 0.06). Gibbium aequinoctiale yielded similar results except the entire relationship was shifted up the ordinate to reflect their higher water loss rate and the inflection occurred at 54° C rather than at 50°C for M. affine (data not presented).