Journal of Insect Science: Vol. 9 | Article 24

Table 3. Larval survival, egg viability, and instar development of F1 progeny from WCRL and Bt4R reciprocal crosses.

Moth pair¹		Total No. viable eggs	% egg viability	No. developed to instar in 21 d²			Mean proportion developed to ≥4th instar³	Corrected % Survival⁴
♀	♂	Total No. viable eggs	% egg viability	2^nd	3^rd	4^th	Mean proportion developed to ≥4th instar³	Corrected % Survival⁴
Reared on untreated artificial diet
Bt4R	WCRL	298	54.7 a⁵	0	2	97	0.42 ± 0.013 a⁶	-
WCRL	Bt4R	897	80.3 a	0	1	232	0.29 ± 0.091 a	-
Bt4R	Bt4R	681	69.1 a	0	0	179	0.31 ± 0.073 a	-
WCRL	WCRL	1284	82.6 a	0	3	330	0.26 ± 0.082 a	-
Reared on artificial diet with 10 μg Cry1Ac per milliliter diet
Bt4R	WCRL	414	59.7 a	47	1	1	0.0029 ± 0.0029 b	0.74
WCRL	Bt4R	955	76.4 a	38	10	22	0.034 ± 0.027 b	8.9
Bt4R	Bt4R	797	69.8 a	0	2	145	0.23 ± 0.048 a	69
WCRL	WCRL	1556	84.7 a	36	11	0	0.0 ± 0.0 b	0.0

¹Reciprocal crosses were performed by mixing five females with five males from each strain in appropriate combinations and were replicated five times.

²No 1^st instar larvae were found on untreated or Cry1Ac-treated diet. Fourth instar larvae category also includes those insects that developed to the pupal stage.

³Mean proportion ± SE was determined by calculating the number that survived to 4th instar larvae or pupae divided by the total number of viable individuals and calculating the mean for each of the five replicates.

⁴Corrected % survival was calculated as survival on Cry1Ac-treated diet divided by survival on untreated diet multipled by 100%. Only live 4th instars or pupae at 21 d after the start of bioassay were counted as survivors.

⁵Mean proportion of egg hatch from each cross were not significantly different (α=0.05) as determined by ANOVA.

⁶Mean proportion of larvae that developed to 4th instar or pupae with the same letter were not significantly different (α=0.05) as determined by the Student-Newman-Keuls multiple comparison test.