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Supplemental Data 2. Charactersistics of the five Tuberculatus quercicola and Formica yessensis microsatellite loci in each tree.
Tree
1 2 3 4 5 6
Locus n nall/h He/Ho n nall/h He/Ho n nall/h He/Ho n nall/h He/Ho n nall/h He/Ho n nall/h He/Ho
Tq15 97 10/0.81 78.8/33* 81 7/0.59 47.8/29* 63 9/0.51 32.5/23 62 10/0.75 46.6/11* 45 10/0.59 39.1/11* 14 2/0.13 1.9/0
Tq17 97 2/0.30 28.8/25 81 2/0.49 39.9/37 63 2/0.49 31.0/27 62 2/0.50 31.2/32 45 2/0.49 14.6/14 14 2/0.38 5.4/7
Tq18 97 2/0.50 48.7/51 81 2/0.46 37.7/37 63 2/0.33 20.8/26 62 2/0.49 30.4/26 45 2/0.46 6.5/3 14 2/0.13 1.9/0
Tq23 97 4/0.66 65.0/86* 81 5/0.73 59.7/71 63 5/0.73 46.5/60* 62 7/0.75 46.5/60 45 5/0.73 33.6/38 14 5/0.75 10.9/10
Tq26 97 4/0.47 46.0/43 81 4/0.52 42.0/44 63 4/0.60 38.4/37 62 3/0.51 31.6/32 45 4/0.52 28.6/28 14 3/0.52 7.5/8
Fy3 80 5/0.59 47.4/47 75 5/0.67 50.5/56 72 5/0.66 47.6/54 58 6/0.62 36.2/35 61 7/0.58 35.9/32 29 6/0.59 17.5/19
Fy4 80 2/0.49 39.7/43 75 2/0.50 37.7/41 72 2/0.47 33.7/39 58 2/0.50 29.2/23 61 2/0.49 30.3/34 29 2/0.13 3.8/2
Fy7 80 6/0.81 65.5/60 75 8/0.84 63.2/55 72 7/0.80 58.3/53 58 8/0.70 41.2/41 61 7/0.71 43.7/26* 29 5/0.57 16.9/6*
Fy15 80 5/0.63 50.5/28* 75 5/0.74 55.1/36* 72 5/0.70 50.7/43 58 6/0.67 39.0/32 61 4/0.64 39.5/18* 29 5/0.71 21.0/24
FL12 80 3/0.60 48.2/50 75 3/0.50 37.6/39 72 3/0.44 31.8/35 58 4/0.69 37.8/35 61 3/0.61 37.2/36 29 3/0.60 17.8/21

n indicates the number of allelic genotypes found across the seasons.

nall and h show the number of alleles across the seasons and gene diversity.

He and Ho mean expected frequency of heterozygotes and observed frequency of heterozygotes.

*indicates significance of deviation in expected genotype frequencies from Hardy-Weinberg expectation calculated by Fisher's exact test with a sequential Bonferroni multiple comparisons.

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