Feeding disruption in Myzus persicae by a new insecticide, flonicamid
FMC Corporation, Princeton, NJ, USA
Correspondence: bruce_black@fmc.com
An important feature of a piercing/sucking insecticide is its ability to rapidly inhibit feeding. An assay was developed to measure feeding cessation in aphids. 14C-Inulin is a non-metabolizable sugar that can be co-administered along with an insecticide in the aphid diet using feeding chambers. Honeydew was quantitatively collected from the lids of the feeding chambers and quantified. Aphids were introduced into chambers and allowed 36 hours to acclimate and begin feeding. At zero time, the diet was changed to a diet containing 14C-Inulin as well as the test compound administered at 100X the calculated LD50. The experiment was performed two ways: Cumulative honeydew was monitored (i.e. from time=0 to time point) and as rates/hour collected as timed intervals collected during the study. Both approaches gave essentially identical results. Feeding behavior stops immediately (extrapolated to t=0) for flonicamid upon insecticide exposure. On the other hand, mortality did commence until 35 hours of exposure (t1/2=40 hours). Honeydew recovered from treated aphids was evaluated for oligosaccharide composition. The ability to form complex polysaccharides was not affected during intoxication.
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