Measuring genetic variation of tarnished plant bug, Lygus lineolaris (Palisot de Beauvois), over temporal and spatial scales
Southern Insect Management Research Unit, USDA-ARS, Stoneville, MS 38776, USA
Correspondence: op.perera@ars.usda.gov
Five Lygus lineolaris populations were collected over a six month period along approximately 10 mile transect in Washington County, MS. Collections made in May, July, and September, 2006 were analyzed using 14 polymorphic microsatellite loci. Allele frequencies were used to estimate the Nei's genetic diversity parameters. The total genetic diversity (Ht), the within population genetic diversity (Hs), the among population genetic diversity (Dst) and the coefficient of genetic differentiation (Gst) estimates were 0.485, 0.453, 0.032, and 0.066, respectively. Overall gene flow estimates, calculated using private allele method, between populations in May, July, and September were 2.66, 12.96, and 16.43, respectively. The data suggest minimal genetic differentiation among populations in the study area and a correlation between gene flow and increase in population size.
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