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Table 5. BestKeeper descriptive statistical analysis for reference genes based on cycle threshold (Ct) values
actin RPS18 GAPDH αTUB RPL13a UBC B.I. B.I.a B.I.b B.I.c
Data for 6 candidate reference genes 4 genes 3 genes 2 genes
n 20 20 20 20 20 20 20 20 20 20
G.M. [Ct] 16.54 14.34 15.06 20.15 18.16 19.28 17.12 16.34 15.29 15.40
A.M. [Ct] 16.60 14.36 15.13 20.22 18.26 19.37 17.18 16.39 15.33 15.44
Min [Ct] 15.05 12.85 13.40 18.35 15.60 16.65 15.65 15.12 14.14 14.09
Max [Ct] 20.10 16.00 18.65 24.95 22.40 22.90 20.41 19.67 18.17 17.93
S.D. [± Ct] 1.21 0.72 1.23 1.51 1.67 1.61 1.23 1.07 1.01 0.91
C.V. [% Ct] 7.29 5.00 8.14 7.48 9.17 8.31 7.17 6.54 6.59 5.93
Min [x-fold] −2.82 −2.80 −3.17 −3.47 −5.89 −6.26 2.72 2.31 2.17 2.51
Max [x-fold] 11.76 3.17 12.00 27.93 18.91 12.53 9.29 9.64 7.02 5.91
S.D. [± x-fold] 2.31 1.65 2.35 2.85 3.19 3.05 2.31 2.08 1.98 1.90
ranking 2 1 3 4 6 5

a, b, cBestKeeper index computed together with the same descriptive parameters for respectively 4 genes (RPS18, actin, GAPDH and αTUB), three genes (RPS18, actin and GAPDH) and two genes (RPS18 and actin)
Abbreviations: n = number of samples; G.M. [Ct] = geometric mean of Ct; A.M. [Ct] = arithmetic mean of Ct; Min [Ct] and Max [Ct] = extreme values of Ct; S.D. [± Ct] = standard deviation of the Ct; C.V. [%Ct] = coefficient of variance expressed as a percentage on the Ct level; Min [x-fold] and Max [x-fold] = extreme values of expression levels expressed as absolute x-fold over or under coefficient; S.D. [± x-fold] = standard deviation of the absolute regulation coefficients; B.I. = BestKeeper index
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