Journal of Insect Science: Vol. 8 | Article 33

Table 1. Information of the primers used for real-time PCR

Gene	Genbank accession number	Primer sequence (5′ to 3′)	Amplicon length (bp)	Ta^a (°C)	% Identity^b	E^c (%)	R²^d
actin	AB023025	TGCCAACACTGTCCTTTCTG	155	61	99%	105.7	0.996
		AGAATTGACCCACCAATCCA
αTUB	XM_396338	AGCATTCAGATTGCGCTTTT	141	61	99%	105.0	0.997
		GCAACGACGCTGTTATTGAA
GST1	AY620822	AATTTGATGAACGGGGAACA	135	61	98%	n.d.	n.d.
		GTATTGGTCCGCCAAGTACG
GAPDH	XM_393605	GATGCACCCATGTTTGTTTG	203	61	100%	75.0	0.991
		TTTGCAGAAGGTGCATCAAC
HMBS	XM_624258	TGGATCTCGAAAAAGCGAGT	207	61	97%	64.6	0.993
		AAATCAACACGGCCACTTTC
RP49	NM_001011587	TGTGCTGAAATTGCTCATGG	104	54	97%	n.d.	n.d.
		CGTAACCTTGCACTGGCATA
RPL13a	XM_623810	TGGCCATTTACTTGGTCGTT	191	61	98%	96.5	0.990
		GAGCACGGAAATGAAATGGT
RPS18	XM_625101	GATTCCCGATTGGTTTTTGA	149	61	97%	102.9	0.998
		CCCAATAATGACGCAAACCT
SDHA	XM_623062	AGCAGGATTACGTGCAGCTT	124	61	96%	144.4	0.988
		ACCCAAGGCAGCATTGATAC
UBQ	XM_393173	GGAGGTTGAAGCATCCGATA	200	61	97%	100.4	0.997
		TGACGAAAATTTGCATTCCA
TBP	XM_623085	TTGGCAGCAAGAAAGTATGC	100	56	100%	n.d.	n.d.
		TCACATCACAGCTGCCTACC

^aOptimal annealing or elongation temperature in the PCR program for a specific primer set
^bPercentage sequence identity between the amplicon and the corresponding reference sequence from Genbank
^cMeasure of the real-time PCR reaction efficiency (calculated by the standard curve method)
^dReproducibility of the real-time PCR reaction
n.d. = no data; gene was excluded from the study