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Table 1. Primers used to amplify polygalacturonase and loading control (putative identifications based on homology) genes complementary and genomic from Lygus lineolaris DNA.
Gene or EST Primer description Location Sequence (5′-3′)
Llpg1 Initial RACE, for 5′ end and genomic intron 103-81 CCTGTTTGGCAGCACCCAGTTGA
Llpg1 Nested RACE, for 5′ end 65-41 ACGGCACCGTAAGCAGATACGATGA
Llpg1 Initial RACE, for 3′ end 891-915 TATCGTCTTCAACAACGTGCGAGGA
Llpg1 Nested RACE, for 3′ end 986-1010 GCAACATTAACGTCACTGGAGGTCA
Llpg1 RT-PCR, forward and full-length/proofread (17)-1 GACAAACGGGCTGTGAATA
Llpg1 RT-PCR, reverse 1150-1131 AGGGGACAAATCAAGGCTCT
Llpg1 Full length/proofread 1216-1194 TTTTTTTTTTTGGCTTCAAATTG
Llpg1 Genomic intron, forward (847)-(826) TGCCAAAACTGACTGCTATTCC
Llpg2 Initial RACE, for 5′ end 142-118 GGACTTGGAGGTCTCTGATGACGAT
Llpg2 Nested RACE, for 5′ end 46-22 CAACCACGAAGAACAAGCACCCAAG
Llpg2 Initial RACE, for 3′ end 863-887 ACCCAACTGGTGAGCCAACATTCTT
Llpg2 Nested RACE, for 3′ end 978-1003 TGCTAGGAACTGGAAGTGGGAAATG
Llpg2 RT-PCR, forward and full-length/proofread (27)-(10) GTTAGCAGCCCCAGAAGT
Llpg2 RT-PCR, reverse 1084-1068 CATCGGATTCCAAGATTC
Llpg2 Full-length/proofread 1126-1104 CAATGAATAACATCTGAGTGACG
Llpg3 Initial RACE, for 5′ end 176-153 GTTCCTGGCTTGACGAATTCAAAA
Llpg3 Nested RACE, for 5′ end 42-20 TGCTGCGACGCACATCAAAATTC
Llpg3 Initial RACE, for 3′ end 650-673 TGGCAGTTGGAGGATACCAAGTGA
Llpg3 Nested RACE, for 3′ end 800-821 AGGACGTCACTGATGCTGGACT
Llpg3 RT-PCR, forward and full-length/proofread (24)-(7) AGGTTTCTGAACCCCAAA
Llpg3 RT-PCR, reverse and full-length/proofread 1109-1090 TTTTGTCTGCCTGTATGATG
ribosomal S2 RT-PCR loading control 256-373 CCGGTCAGAGAACCAGAT
ribosomal S2 RT-PCR loading control 701-684 GTACGGAGGCTTGGAGAG
ribosomal 16S RT-PCR loading control 717-734 GAAGGCTGGTATGAATGG
ribosomal 16S RT-PCR loading control 1046-1029 TCGCAAACTTTCTTATCG
actin RT-PCR loading control 578-597 CGATCTACGAGGGATACGCT
actin RT-PCR loading control 967-948 ACAGGTCCTTACGGATGTCC
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