Figure 4a. ClustalW 1.81 multiple sequence alignment of the deduced amino acid sequence of the putative rifampicin resistance protein homologs from Amsacta moorei entomopoxvirus (AmEPV), Heliothis armigera entomopoxvirus (HaEPV), Melanoplus sanguinipes entomopoxvirus (MsEPV), Molluscum contiguosum poxvirus (MOLCV), swinepox virus (SPV), Myxoma poxvirus (MYXV), vaccinia virus (VACV), variola virus (VARV), and Diachasmimorpha longicaudata entomopoxvirus (DlEPV). A colon (:) represents amino acid homologous (“conservative”, sensu Mount 2001) substitutions. A period (.) identifies amino acid non-homologous substitutions. Asterisks indicate identical amino acids conserved in all sequences. Underlined sequences represent regions I and II in HaEPV and DlEPV with the highest percent conserved amino acids previously identified for HaEPV by Osborne et al. (1996). For the three motifs identified within the RIF sequence, Blue = conserved in all poxviruses; Red = conserved only among chordopoxviruses; Green = conserved only among EPVs. Other colors = conserved in some members of a subfamily.