Fourth International Bemisia Workshop International Whitefly Genomics Workshop
Gene expression in pyriproxyfen resistant Bemisia tabaci Q biotype
Institute of Plant Protection, Department of Entomology, the Volcani Center, Bet Dagan, Israel. Correspondence: ghanim@agri.gov.il
Pyriproxyfen is a biorational insecticide that acts as a juvenile hormone analog (JHA) and disrupts insect development with unknown molecular mode of action. This insecticide is one of the major insecticides being used to control the whitefly Bemisia tabaci (Gennadius), and is one of the major insecticides that comply with integrated pest management (IPM) programs, because of minimal effects on the environment, humans and beneficial organisms. During the last few years, resistance to pyriproxyfen has been observed in several locations in Israel; sometimes reaching thousand-fold increase in the resistance. The molecular basis underlying this resistance, which may lead to understanding the mode of action of pyriproxyfen, and developing molecular markers for rapid monitoring of resistance outbreaks, was not studied. In this communication, we used a recently developed cDNA microarray from B. tabaci to monitor changes in gene expression in resistant B. tabaci populations. Based on statistical analysis we identified 111 expressed sequence tags (ESTs) that were differentially regulated in the resistant strain. Although many of the regulated ESTs observed in our study belong to families usually associated with developed resistance and xenobiotic detoxification such as mitochondrial genes, P 450s and oxidative stress, many other genes associated with protein, lipid and carbohydrate metabolism, and genes related to JH associated processes in insects such as oocyte and egg development were also observed.
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