Table 1. Purification of PBGS from P. brassicae.
| Total activity* | Total protein | Specific activity* | Purification | Yield | |
|---|---|---|---|---|---|
| Purification step | (μmol/h) | (mg) | (μmol h −1 mg −1 ) | (fold) | (%) |
| 1. Heated extract | 117.8 | 4173 | 0.028 | 1 | 100 |
| 2. Ammonium sulfate | 82.9 | 1860 | 0.045 | 1.6 | 70.4 |
| 3. PEG-6000 | 77.6 | 498 | 0.156 | 5.6 | 65.9 |
| 4. TEAE-cellulose | 80.3 | 8.8 | 9.1 | 323 | 68.2 |
| 5. HA-Ultrogel | 72.2 | 3.8 | 19 | 678 | 61.3 |
| 6. Prep. PAGE 1 | 54.9 | 1.1 | 49.9 | 1782 | 46.6 |
| 7. Prep. PAGE 2 | 26.3 | 0.095 | 276.8 | 9886 | 22.3 |