Figure 3. Determination of the molecular mass of PBGS from P. brassicae. A - Native PAGE. The reference proteins were bovine serum albumin (1), citrate synthase (2), lactate dehydrogenase (3), aldolase (4), pyruvate kinase (5) and urease (6). A molecular weight of 292’300 was obtained for the native complex of PBGS. B – SDS/urea-PAGE. The reference proteins were phosphorylase B (1), bovine serum albumin (2), ovalbumin (3), carboanhydrase (4), trypsine inhibitor (5) and lactalbumin (6). A molecular weight of 36’500 was obtained for the subunits of PBGS.