Figure 2. Dependence of PBGS activity on (A) temperature (in 100 mM sodium phosphate, 10 mM 2-mercaptoethanol; pH 6.8) and (B) on pH of phosphate buffer (100 mM sodium phosphate, 10 mM 2-mercaptoethanol) and imidazole buffer (100 mM imidazole/HCl, 2 mM DTT), respectively, both assayed at 50°C.