Figure 1. Analysis by native PAGE of the purification of PBGS from P. brassicae. A - After chromatography on HA-Ultrogel; 5.25% gel. B - After preparative PAGE 1 (slab gel); 7.5% gel. C - after preparative PAGE 2 (rod gel): gel concentrations were 5% (C₁), 6.25% (C₂) and 7.5% (C₃), respectively. In C, each gel was loaded with 10 μg of purified PBGS. Gels A-C were stained with Coomassie Brilliant Blue G-250. The arrows in A and B mark the PBGS band identified by in situ incubation of the gels with substrate followed by product staining with Ehrlich’s reagent, as shown in D (7.5% gel).