Table 2. Effect of electroporation conditions on pupal mortality, and on the adult wing of Bicyclus anynana. None of these experiments used Flyfectin. Animals in rows number 1–6 were injected with DNA whereas animals in row number 7 were injected with water.
| Conditions | Heat-shock duration (hr) | Age @ injection (hr) | Total # operated wings | Total # emerged | % Emerged | % Unexpanded wings (from total emerged) | % Hole in wings and/or ectopic scales | % No effect | |
|---|---|---|---|---|---|---|---|---|---|
| 1) | 80V, 0.1ms, 5 pulses, 100ms interval | 3.5 | 13.0 – 17.5 | 14 | 13 | 93 | 31 | 61 | 8 |
| 2) | 80V, 100ms, 10 pulses, 100ms interval | 3.5 | 12 – 16 | 25 | 22 | 88 | 45 | 28 | 27 |
| 3) | 160V, 0.050ms, 5 pulses, 100ms interval | 5.5 | 12 – 17.75 | 8 | 5 | 63 | 40 | 60 | 0 |
| 4) | 80V, 0.5sec, 3 pulses, 500ms interval | 3 | 6.5 – 14.5 | 30 | 27 | 90 | 33 | 56 | 11 |
| 5) | 80V, 1 sec, 3 pulses, 500ms interval | 3 | 5.75 – 14.5 | 28 | 23 | 82 | 43 | 57 | 0 |
| 6) | 80V, 1.5 sec 3 pulses, 500ms interval | 3 | 12.5 – 16.5 | 30 | 30 | 67 | 56* | 39 | 6* |
| 7) | 80V, 0.5sec, 3 pulses, 500ms interval | 3 | 0.5 – 21.5 | 12 | 10 | 83 | 20 | 80 | 0 |
| 7) | 80V, 1sec, 3 pulses, 500ms interval | 3 | 5.5 – 22.5 | 18 | 16 | 88 | 19 | 68 | 13 |
| 7) | 80V, 1.5sec, 3 pulses, 500ms interval | 3 | 7.5 – 18 | 14 | 11 | 79 | 27 | 63 | 9 |