Table 1. Effect of different electroporation conditions on the expression of the plasmid pBac {3xP3-Dsred, hsp70-EGFP} in the pupal forewing of Bicyclus anynana. Numbered rows (1–7) can be compared with similarly marked rows in Table 2.
| Transfection Conditions | DNA concentration (ng/μl) | Heat shock duration (hr) | Age @ injection (hr) | Hours until dissection | # Wings GFP+ (%) | Average of # cells GFP+ (range) | SD | |
|---|---|---|---|---|---|---|---|---|
| 1) | 80V, 0.1ms, 3–10 pulses, 100ms interval | 940 | 2 – 3.5 | 4.5 – 19.5 | 4.5 – 6 | 2/22 (9) | 2505 (10 – 5000) | 3528 |
| 2) | 80V, 100ms, 10 pulses, 100ms interval | 940 | 2.5 – 3.5 | 3.5 – 18.75 | 1.5 – 26 | 12/51 (24) | 140 (10–500) | 129 |
| 3) | 160V, 0.05ms, 5 pulses, 100ms interval | 940 | 5.5 | 12 – 16.25 | 3.5 – 4 | 0/6 (0) | 0 (0) | 0 |
| 160V, 100ms, 10 pulses, 100ms interval | 940 | 3 – 3.5 | 10.5 – 16.5 | 5 – 26 | 0/15 (0) | 0 (0) | 0 | |
| 80V, 0.5 sec, 3 pulses, 500ms interval (+ Flyfectin) | 550 – 570 | 3 | 4.5 – 14.5 | 24 – 27 | 5/18 (28) | 246 (10 – 1000) | 301 | |
| 4) | 80V, 0.5sec, 3 pulses, 500ms Interval | 550 –570 | 3 | 5 – 12.5 | 23 – 25 | 5/20 (25) | 237 (10 – 1000) | 308 |
| 80V, 1 sec, 3 pulses, 500ms interval (+ Flyfectin) | 550 – 570 | 3 | 4.25 – 16 | 21 – 25 | 15/22 (68) | 676 (10 – 2000) | 644 | |
| 5) | 80V, 1 sec, 3 pulses, 500ms interval | 550 – 570 | 3 | 3.25 – 15 | 22 – 23 | 7/19 (37) | 972 (20 – 3000) | 843 |
| 80V, 1.5 sec 3 pulses, 500ms interval (+ Flyfectin) | 550 – 570 | 3 | 7.5 – 14.5 | 24 – 26 | 9/20 (45) | 271 (20 – 1000) | 289 | |
| 6) | 80V, 1.5 sec, 3 pulses, 500ms Interval | 550 – 570 | 3 | 10 – 14.5 | 23 – 25 | 8/20 (40) | 387 (20 – 2000) | 512 |
| 7) | Control: 3 animals from each of the conditions above | 0 | 2–5.5 | 3.25–19.5 | 1.5–27 | 0/30 (0) | 0 (0) | 0 |