Figure 3. Immunolocalization of vitellogenin in a stage 3, vitellogenic oocyte; primary magnification of 40 x (a-b, corresponding controls (primary antibody substituted with buffer) c-d). The inside of the vitellogenic oocyte is covered in a grayish area of labeled antigen. The arrows point to areas where vitellogenin (gray or black dots) seems to be transported through pores in the follicle cells (a-b) or directly through the follicle cells in granules to the perivitelline space (see arrows) and thus, seems to be supporting already existing data (Fleig 1995). The controls (c-d) show no positive staining for vitellogenin.