Figure 2. Vitellogenin staining of a stage 3, vitellogenic ovary with developing oocytes.In a and b, the immunogold stained ovary (right) with corresponding control (left) is recorded at 16 x. The images consist of a mosaic of pictures. In controls, primary antibody was substituted with buffer. The silver enhanced immunogold staining of vitellogenin shows as black dots in bright field pictures. At 16 x magnifications the separate black dots are not easily detected and appear as a black or grayish area of stain. The ovarioles consists of clusters of trophocytes (t) connected to one oocyte alternately in anterior-posterior direction. The vitellogenic oocytes (vo) appear to incorporate proteins in granules and are intensely labeled for vitellogenin (b). Some oocytes have irregular shape and may be in process of reabsorption (indicated by arrows in a and b). Abbreviations in the controls.