Figure 2. A-H. Salivary gland cells in different stages of programmed cell death. A. Orange-stained nucleolus (Nu) and green-stained chromosomes in a third instar larva cell stained with acridine orange technique. B. Advanced stage of chromosome alteration; the chromosomes remain green with acridine orange technique. C, D. Cells in very advanced stage of programmed cell death: the new nucleolar body (arrows) is associated with green-stained remains of chromosome material in two disrupted cells (C), or associated with the nuclear membrane (D). E-H. Cells also in very advanced stage of programmed cell death, stained with acridine orange (E, G) and with Ag-Nor (F, H). The arrows point to the same nucleolar bodies in slides stained with the two techniques. In H, the highlighted nucleolus is associated with fine, silver stained threads. In C to H, the cytoplasmic material looks very dense. Scale bars: A= 5.0 μm; B, C, D = 7.2 μm; E-H= 11.5 μm.