Figure 1. Measurement of EF-1α levels in larval tissues of Spodoptera frugiperda by ELISA. (A) A standard curve was generated by incubating Mab 7D6 with two-fold dilutions of purified EF-1α, plotted as ng/well versus absorbance at 410 nm. Data points (open circles) ± SD (error bars) and the resultant regression line (r² = 0.997) are shown. Values for the slope (m) and y-intercept (b) of the line are given. (B) Tissue concentrations of EF-1α are presented as a percent of the total protein in each tissue (open bars) ± SD (error bars). Statistically different EF-1α concentrations are indicated by different letters (P<0.01, 1-way ANOVA and Tukey test). The tissues examined were fat body (FB), Malpighian tubules (MT), midgut (MG), muscle (MU), salivary gland (SG), trachea (TR), and ventral nerve cord (VNC).