Figure 1. Gel of detected virus from salivary gland (Sg) and midgut (Mg) tissues of Homalodisca coagulata adults tested for presence of HoCV-1, using rtPCR. Both types of tissues from individual insects were dissected and analyzed in a pairwise fashion. Only midgut tissues were shown to test positively for virus presence. M = ladder wide-range DNA marker (16 fragments 50-10,000 bp), Sg = salivary glands, Mg = midgut tissue. Amplified fragment ~ 443 bp, was validated by sequencing of the amplified product and Blast analysis.