Figure 1. Embryonic developmental of the pleuropodium of Magicicada cassini as revealed by anti-HRP immunhistochemistry. The embryonic development has been staged in percent according to that of Schistocerca nitens (Bentley et al. 1972). A; Brightfield micrograph of an embryo of 30–35% of embryonic development. ab: abdomen (the distal segments are missing), h: head, le: leg anlagen, th: thorax. B to I; Anti-HRP immunhistochemistry of the different developmental stages of pleuropodia. a1-a3: abdominal segments 1 – 3, cr: crescent, do: dome, pe: pedunculus, t3: metathoracic segment. The arrows point to the pleuropodia in the first abdominal segment. Scales: A: 100μm, B,C,F: 50μm, D,E,G,H,I: 20μm.