Figure 5. Northern blot analysis. The numbers 0, 2, 4, 6, and 12 underneath the figure represent samples extracted from 0-day (freshly hatched), 2-day, 4-day, 6-day, and 12-day old larvae, respectively, while the letters P and A represent samples extracted from pupae and adults. A blot was hybridized to a probe derived from the SSGP-11A1 gene (11A1). After the radioactivity stripped or decayed (no image by 48 hours exposure), the same blot was rehybridized sequentially to probes specific to SSGP-11B1 (11B1), SSGP-11C2, and SSGP-12A1, an unrelated SSGP gene (Liu et al. 2004). An image of the 18 S rRNA for the blot was included to show that the loading amount of RNA in each lane was about the same.