Figure 7 Phosphorylation of bMBD2/3 in vitro. The reaction mixture containing bMBD2/3 proteins and protein kinases [lane 1, Protein kinase A; lane 2, protein kinase C; lane 3, Ca/CaM dependent protein kinaseII; lane 4, brain extract; lane 5 brain extract (-bMBD2/3)] were incubated with [-32P]ATP. The samples were treated with SDS–sample buffer. After electrophoresis, the gel was analyzed using a bioimaging analyzer (upper lanes). The bands stained with Coomassie Brilliant Blue after electrophoresis (lower lanes).