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Table 2. Microsatellite markers previously published for Helicoverpa armigera found to unsuccessfully amplify similar microsatellite loci in three other related lepidopteran species.
Species Locus Annealing temperature (C0) - DNA band fragment profile Number of replicates Sample size PCR running conditions
H. zea HaD47 500 - single-copy 240 bp band 1 2 Scott et al., 2004; Tan et al. 2001 (modified)
550 - multiple bands 3 18
H. zea Ham3 530 - multiple bands 1 6 Tan et al., 2001; Tan et al. 2001 (modified)
600 - multiple bands > 200 bp marker 1 6
660 - multiple bands > 200 bp marker 2 12
H. zea HarSSR2 520 - fb 1 2 Scott et al., 2004; Tan et al. 2001 (modified)
600 - nb 1 4
H. zea HarSSR3 590 - nb 1 2 Scott et al., 2004; Tan et al. 2001 (modified)
600 - multiple bands 1 4
550 - multiple bands > 200 bp marker 1 3
H. zea HarSSR4 60 - single-copy 240 1 2 Scott et al., 2004; Tan et al. 2001 (modified)
660 - multiple bands > 240 bp band 1 3
H. zea HarSSR5 660 - multiple bands 1 4 Tan et al., 2001
H. zea Ham5 520 -multiple bands 1 2 Tan et al., 2001
H. virescens HaC87 500 - multiple bands 1 2 Scott et al., 2004; Tan et al. 2001 (modified)
550 - multiple bands 1 4
600 - nb 1 4
H. virescens HarSSR2 520 - fb 1 2 Tan et al. 2001(modified); Scott et al., 2004
660 - multiple bands 2 7
H. virescens HarSSR3 590 - multiple bands 1 2 Scott et al., 2004
600 - multiple bands 1 3
500 - multiple bands 2 7
H. virescens HarSSR4 600 - multiple bands 1 2 Scott et al., 2004
530 - multiple bands 1 2
H. virescens Ham3 550 - multiple bands 1 4 Tan et al., 2001
H. virescens Ham5 520 - multiple bands 1 2 Tan et al., 2001
550 - nb 1 4
H. virescens Ham6 660 - nb 1 3 Tan et al., 2001
H. subflexa HaC87 500 - multiple bands 1 2 Scott et al., 2004
550 - multiple bands > 200 bp marker and strongly stained bands at 55, 70, 75 bp markers 2 18
H. subflexa HaD47 500 - multiple band 1 2 Scott et al., 2004
550 – suspected single-copy band at 140 bp 2 10
H. subflexa HaD47 500 - multiple bands 1 2 Scott et al., 2004
H. subflexa Ham3 530 - multiple bands >180 bp marker band 2 16 Tan et al., 2001; Tan et al. 2001 (modified)
590 - multiple bands 1 4
H. subflexa Ham6 530 - multiple bands 1 4 Tan et al., 2001
H. subflexa HaSSR2 520 - nb 1 2 Tan et al., 2001
H. subflexa HaSSr3 590 - fb 1 2 Tan et al., 2001
H. subflexa HaSSR4 600 - fb 1 2 Tan et al., 2001
H. subflexa HaSSR5 540 - fb 1 2 Tan et al., 2001
nb= no bands detected; fb = faint bands
Repetitive sequences are defined as repeated genomic regions containing microsatellite motifs and their flanking regions.
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