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Table 1. Microsatellite markers previously published for Helicoverpa armigera found to successfully amplify similar microsatellite loci in three other related lepidopteran species.
Species Locus Annealing temp (C0) - DNA band fragment profile Number of replicates Sample size (n) Reference for PCR running conditions
H. zea HaB60 50° - multiple bands > 200 bp marker 1 7
55° - single160 bp band 3 18 Tan et al., 2001(modified)*
60° - multiple bands > 200 bp marker and a single 160 bp band 2 12
H. zea HaC87 50° - single-copy band 118 bp band 2 14 Scott et al., 2004; Tan et al., 2001(modified)
55°- multiple bands 1 3
60° - single-copy 118 bp band 2 12
H. zea HaC14 50° - single-copy 160 bp band 1 12 Scott et al., 2004
55° - single-copy 160 bp band 1 3 Tan et al., 2001(modified)
H. zea HarSSR1 50° - single-copy 240 bp band 1 2 Scott et al., 2004
58° - single-copy 240 bp band 2 5
50° - multiple bands 2 12
H. virescens HaC14 550 - single-copy 160 bp 2 15 Scott et al., 2004
50° - multiple bands 2 4 Scott et al., 2004
H. virescens HaB60 55° - 160 bp and 140 bp single-copy bands 3 11 Tan et al., 2001(modified)
50° - 240 bp single-copy band 1 5
H. virescens HarSSR1 58° - multiple bands 2 4 Scott et al., 2004
60° - multiple bands 1 2
H. subflexa HaB60 50° - multiple bands 2 4 Scott et al., 2004
55° - 160 bp and 140 bpsingle-copy bands 3 14
H. subflexa HaC14 50° - 160 bp single-copy band 3 14 Scott et al., 2004
55° - multiple bands 1 2
H. subflexa HaSSR1 58° - 240 bp single-copy band 2 4 Scott et al., 2004
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