Table 1.
| Year | Host | Dissection Results | Polymerase Chain Reaction | Endonuclease Digestion | ||||||
| Nymphs1 | Parasitism2 | Larvae3 | Peristenus4 | Other5 | Unamplifiable6 | P. howardi7 | P.pallipes8 | Unknown9 | ||
| 2002 | Parma alfalfa10 | 575 | 267 | 20 | 20 | 0 | 0 | 18 | 0 | 2 |
| Alfalfa hay | 390 | 7 | 3 | 3 | 0 | 0 | 3 | 0 | 0 | |
| Alfalfa seed | 462 | 56 | 23 | 18 | 4 | 1 | 18 | 0 | 0 | |
| Clover seed | 590 | 34 | 28 | 24 | 3 | 1 | 21 | 1 | 2 | |
| Kochia | 160 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | |
| Pepperweed | 1379 | 85 | 57 | 34 | 10 | 13 | 31 | 0 | 3 | |
| Hoary cress | 257 | 3 | 3 | 2 | 0 | 1 | 1 | 0 | 1 | |
| Feral alfala | 327 | 55 | 35 | 30 | 3 | 2 | 30 | 0 | 0 | |
| Total | 4140 | 509 | 170 | 131 | 21 | 18 | 122 | 1 | 8 | |
| 2003 | Parma alfalfa10 | 2018 | 725 | 62 | 46 | 14 | 2 | 42 | 0 | 4 |
| Alfalfa hay | 979 | 12 | 12 | 11 | 1 | 0 | 8 | 0 | 3 | |
| Alfalfa seed | 1799 | 83 | 78 | 65 | 8 | 5 | 62 | 2 | 1 | |
| Clover | 893 | 28 | 28 | 25 | 2 | 1 | 25 | 0 | 0 | |
| Kochia | 219 | 8 | 8 | 7 | 1 | 0 | 7 | 0 | 0 | |
| Pepperweed | 1396 | 172 | 170 | 146 | 20 | 4 | 138 | 0 | 8 | |
| Hoary cress | 775 | 59 | 57 | 45 | 9 | 3 | 43 | 0 | 2 | |
| Total | 8079 | 1087 | 415 | 345 | 55 | 15 | 325 | 2 | 18 | |
| Both years | 12219 | 1596 | 585 | 476 | 76 | 33 | 447 | 3 | 26 | |
1 Total number of Lygus spp. nymphs collected from the respective plant hosts.
2 Number of Lygus spp. nymphs with at least one parasitoid at dissection.
3 Number of parasitoid larvae subjected to polymerase chain reaction.
4 Number of parasitoid larvae that produced an amplimer using Peristenus-specific primers C1-J-2252 and TL2-N-3014.
5 Number of parasitoid larvae that produced an amplimer using universal insect primers 5.9S-F and 28S-R, but not with Peristenus-specific primers; interpreted as non-Peristenus parasitoid species.
6 Number of parasitoid larvae that produced no amplimer with either primer set; interpreted as unamplifiable DNA.
7 Number of Peristenus-specific amplimers that produced an SfcI digestion banding pattern matching that of the known P. howardi reference.
8 Number of Peristenus-specific amplimers that produced an SfcI digestion banding pattern matching that of the known P. pallipes or P. pseudopallipes references, which are indistinguishable using this endonuclease.
9 Number of Peristenus-specific amplimers that produced an SfcI digestion banding pattern not matching any of the Peristenus spp. references.
10 Unreplicated data from Parma Research and Extension Center alfalfa seed field provided for comparison.
2 Number of Lygus spp. nymphs with at least one parasitoid at dissection.
3 Number of parasitoid larvae subjected to polymerase chain reaction.
4 Number of parasitoid larvae that produced an amplimer using Peristenus-specific primers C1-J-2252 and TL2-N-3014.
5 Number of parasitoid larvae that produced an amplimer using universal insect primers 5.9S-F and 28S-R, but not with Peristenus-specific primers; interpreted as non-Peristenus parasitoid species.
6 Number of parasitoid larvae that produced no amplimer with either primer set; interpreted as unamplifiable DNA.
7 Number of Peristenus-specific amplimers that produced an SfcI digestion banding pattern matching that of the known P. howardi reference.
8 Number of Peristenus-specific amplimers that produced an SfcI digestion banding pattern matching that of the known P. pallipes or P. pseudopallipes references, which are indistinguishable using this endonuclease.
9 Number of Peristenus-specific amplimers that produced an SfcI digestion banding pattern not matching any of the Peristenus spp. references.
10 Unreplicated data from Parma Research and Extension Center alfalfa seed field provided for comparison.