Figure 3. Top: HPLC chromatogram of an extract of 60 Leucophaea maderae brains separated on a diphenyl column under similar conditions as the first separation step described in Materials & Methods. Bottom: Immunoreactivity of the minute-fractions separated above as measured with the anti-Drm-PDF antiserum. Only the largest immunoreactive peak revealed pigment-dispersing bioactivity (A), all others were inactive (IA).