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Table 3: Summary of purification of larval carcass DPP IV activity
Enzyme Fraction Total Activity Total Protein (mg) Specific Activity (Abs/mg Protein) % Yield Fold Purification
WT CH 787.5 427.63 1.84 100.00  
WT MF 296.4 86.33 3.43 37.64 1.86
WT MF DEAE 228.8 2.19 104.35 29.05 56.71
WT MF GF 86.6 0.5 171.51 11.00 93.21
WT CF 541.2 367.96 1.47 68.72 0.80
WT CF DEAE 74.2 3.95 18.8 9.42 10.22
WT CF GF 35.18 1.25 28.2 4.47 15.33
Ω CH 70.8 116.39 0.61  
Ω MF 45.38 39.46 1.15 64.09 1.89
Ω MF DEAE 19.95 5.45 3.66 28.18 6.01
Ω MF GF 3.78 0.47 8.06 5.34 13.26
Ω CF 75 86.98 0.86 105.93 1.42
Ω CF DEAE 42.24 3.8 11.13 59.66 18.29
Ω CF GF 5.16 0.39 13.29 7.29 21.84

Table Legend: Abbreviations: MF:Membrane Fraction, CF: Cytosol Fraction, CH: Crude Homogenate, GF: Sample run through the DEAE column then the Gel Filtration Column, DEAE: Extracts run though DEAE column only, WT: Wild-type. Percent Yield was calculated by the total activity at a given step/total activity of the crude extract. Fold purification was calculated as specific activity of a purified sample/specific activity of the crude extract.

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