Figure 6. PCR analysis of several genes in tetracycline-treated Sitophilus oryzae. DNA prepared from rice weevils was analyzed after 2, 4, 5, 6 and 7 weeks of exposure to tetracycline (0.5 µg/g flour). The control (C) is analysis of DNA from the normal rearing of the weevils for 7 weeks in the absence of the drug. The number of cycles was constant for the PCR analysis of DNA prepared after the various times of rearing: 35 cycles for analysis of the Wolbachia 16S-RNA gene and 30 cycles for the other genes (S. oryzae 18S-RNA gene, the SOPE 16S-RNA gene, and the rice weevil pectinmethylesterase gene). Experimental details are given in Shen et al. (2003).