Figure 5. Detection of pectinmethylesterase mRNA in adult Sitophilus oryzae digestive tracts by in situ hybridization. Methods are given in Shen et al. (2003). The control (B) lacked exposure to the digoxygenin-labeled probe. A control lacking the alkaline-phosphatase-coupled antibody was also devoid of signal (not shown). The full complement of reagents (A) gave strong staining stained throughout the midgut (including the ventriculus, the enlarged anterior portion of the midgut) and weak staining in the anterior portion of the hindgut. Abbreviations: hg, hindgut; mg, midgut; pv, proventriculus; py, pylorus; ven, ventriculus.