Figure 4. Phylogram of pectinmethylesterase sequences. The phylogram was created starting with a multiple alignment created with ClustalW version 1.82. The alignment included several sequences in addition to those shown in Figure 3. The additional sequences are: Erwinia chrysanthemi CAA59151; Salmonella typhimurium NP_459765; Ralstonia solanacearum P24791; Xanthomonas campestris NP_637620; Streptomyces avermitilis NP_827553; Bradyrhizobium japonica NP_768634; Bacillus licheniformis YP_093018; Clostridium acetobutylicum NP_349964; Oryza sativa NP_916048 ; Medicago trunculata CAB65290; Zea mays CAA73733; Botryotinia fuckeliana CAC29255. In creating the alignment on which this phylogram is based, we used the entire amino acid sequences of the proteins. The phylogram was generated as described in Methods. The consensus tree that is shown is rooted with the Aspergillus sequence. A number in the phylogram indicates the percentage of trees that included the nearest branch point (in 1000 trees constructed).