Figure 3. Alignment of pectinmethylesterase amino acid sequence from Sitophilus oryzae with representative pectinmethylesterase sequences from bacteria, fungi and plants. Sequences (and accession numbers) are: rice weevil, this paper; Erwinia carotovora YP_048235; Escherichia coli NP_415293; Cochliobolus carbonum ADD43340; Aspergillus oryzae BAA75474; Lycopersicon esculentum CAA52704; Arabidopsis thaliana AAM91523. The sequences were aligned using ClustalW version 1.82 (www.ebi.ac.uk/clustalw). Each aligned sequence was trimmed at the N-terminus and C-terminus, to show the region of highest similarity among the 7 sequences. The rice weevil sequence shown, for instance, begins at residue 47 of the mature protein and ends 5 residues short of the C-terminal residue. Asterisks indicate the presence of identical residues in all sequences; colons represent an intermediate level of similarity at a given position; and periods a lower but still marked level of similarity. (See the ClustalW website for definitions: http://www.ebi.ac.uk/clustalw).