Figure 2. Chromatography of recombinant pectinmethylesterase from Sitophilus oryzae on S-Sepharose at pH 4.6. The sample was the flow-through from a Q-Sepharose chromatography (see Methods). A280 is indicated by circles and A620/min (a measure of pectinmethylesterase activity) by squares. The insert shows SDS polyacrylamide gel electrophoresis of fractions 6 - 8, combined, with the masses (in kDa) of two standards, carbonic anhydrase and ovalbumin. The estimated mass of this band was 40 kDa.