Figure 1. Nucleotide sequence of pectinmethylesterase cDNA clone PE6 from Sitophilus oryzae, with the translated sequence of the protein. The arrow indicates a signal-peptide cleavage site predicted by PSORT (psort.nibb.ac.jp; von Heijne 1986). The underlined portion of the underlined amino acid sequence corresponds to sequence determined on the enzyme purified from rice weevils (Shen et al. 1996; Methods of this paper).