Table 1. Primers used for the RT-PCR detection of atypical guanylyl cyclase transcripts.

1 st round primers 2 nd round primers Fragment size Gyc-88E GGTCTATCCCGAGAATCT GCGAAAAAGGCTCAACAG 1050 bp   CTGATACAGCAGCTCATCC CTACGCATCTCCTCATCC   Gyc-89Da AAGTCCTGTTTAACCGTTAT CATCACTCCTGCAAGGAAT 1100 bp   GCAACTCAAAGAGCACGG GCATTGGAACAAGTCCAT   Gyc-89Db GGATGCTGTGCGTGTGCA GCGTATATGCCTTCCTTAAAT 1100 bp   CTCGAAGAGAACTGTACGC CAGAATGGTCTCCCATTGG   rp49 GTATCGACAACAGAGTCGGTCGC 250 bp   TTGGTGAGCGGAGCGACAGCTGC

All primers are shown 5' – 3', with the upstream primer given first.