Figure 6. In situ hybridization in Drosophila melanogaster embryos showed that Gyc-89Da had a similar expression pattern to Gyc-89Db. A Low magnification image of a stage 17 D. melanogaster embryo stained with the Gyc-89Da probe marking regions shown in B-G. B and C Anterior region of a stage 17 embryo showing expression of Gyc-89Da (B) in cells in the terminal ganglion (arrow) and dorsal ganglion (arrow head, out of focal plane) and Gyc-89Db in the terminal ganglion (C arrow). D and E Lateral margins of a stage 17 embryo showing les cells in thoracic segments 2 and 3 staining for Gyc-89Da (D arrows) and Gyc-89Db (E arrows). F and G Both probes stained a total of 12 cells in the caudal sensory cones of the terminal abdominal segments - Gyc-89Da (F eight cells in plane of focus) and Gyc-89Db (G four cells in focus). H No staining is detectable in the midgut with a probe to Gyc-89Da whereas a probe to Gyc-88E stains several cells in the midgut epithelium (I arrows). In all cases anterior is up. The scale bar represents 200 µm in A and 100 µm in B-I.