Figure 5. Expression of the atypical soluble guanylyl cyclase subunits throughout development. RNA was extracted from the stages shown and cDNA synthesis performed in the presence (+) or absence (-) of reverse transcription. The reaction product was then used as a substrate for PCR amplification with specific primers for Gyc-88E, Gyc-89Da, Gyc-89Db or ribosomal protein 49 (rp49) as described in the materials and methods. The developmental stages tested were mixed age embryos (E), mixed larval stages (L), pupae (P) and adults (A).