Figure 2. RACE-amplification of the VHDL receptor and the 78 kDa protein. Panel A: RACE of the 80 kDa receptor. PCR of the 3'-RACE-library was carried out with primers R1 and the universial primer NUP, while the 5'-RACE was first amplified with primers R4 and UPM (1), followed by re-amplification of the 1600 bp band with nested primers R3 and NUP (2). Panel B: 3'-RACE of the 78 kDa protein. PCR was carried out with primers F2-1 and UPM.