Figure 1. Purification of the VHDL receptor. Panel A: Affinity-purified VHDL receptor (1 µg) appeared homogenous in a 12 % SDS gel. The ligand blot was carried out with FITC-labeled VHDL, and the Western blot with anti-VHDL-receptor antiserum, as described in Materials and Methods. Panel B: The affinity-purified fraction could be further separated into two proteins on a larger 15 % SDS gel: the 80 kDa band is the receptor and less prominent 78 kDa protein. Molecular weight markers (175, 83, 62, 48, 33 kDa) are shown on the far right lane.