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Table 2. The distribution of Ostrinia nubilalis mitochondrial RFLP frequencies and haplotypes in samples from 15 North American collection sites. Frequency of digestion (PCR-RFLP frequency), and number of O. nubilalis with a given haplotype at each collection site (mitochondrial haplotype number) are provided. Note: Maine (ME) and Minnesota (MN) samples were divided into ecotype (ET) for genetic distance estimation (see Table 4).
 
PCR-RFLP frequencies
Mitochondrial haplotype numbers
  
Subpopulation ID (collection site)
 ET
D
H
S
M
w +
D
H
S
M
DM
DS
MS
DH
DHM
Total (N)
1 Oxford, MaineU
UE
100
0
10.41
0
43
0
0
5
0
0
0
0
0
0
48
UZ
100
0
18.75
0
13
0
0
3
0
0
0
0
0
0
16
2 Cape Elizabeth, MaineU
BE
100
0
6.25
0
90
0
0
6
0
0
0
0
0
0
96
BZ
100
0
6.25
4.17
43
0
0
3
2
0
0
0
0
0
48
3 Ithaca, New YorkY (lab colony)
BE
100
0
0
0
48
0
0
0
0
0
0
0
0
0
48
BZ
100
0
0
0
48
0
0
0
0
0
0
0
0
0
48
4 Newark, DelawareR
 BE
97.92
2.08
2.08
2.08
46
0
0
1
0
0
0
0
0
1
48
5 Colombia City, IndianaS
 BZ
97.96
3.06
2.04
2.04
90
1
2
2
2
0
0
0
1
0
98
6 Franklin Co, IndianaS
 BZ
100
2.35
1.18
3.53
79
0
2
1
3
0
0
0
0
0
85
7 Crawfordville, Iowa
 BZ
96.88
6.25
2.08
0
85
3
6
2
0
0
0
0
0
0
96
8 Hubbard, Iowa
BZ
100
7.29
2.08
2.08
86
0
7
1
1
0
0
1
0
0
96
9 Kanawa, Iowa
 BZ
100
2.35
1.18
3.53
79
0
2
1
3
0
0
0
0
0
85
10 Mead, NebraskaX
 BZ
98.96
8.33
3.13
1.04
82
1
8
3
1
0
1
0
0
0
96
11 Garden City, KansasT
 MZ
96.47
4.71
4.71
5.88
81
2
4
4
4
1
0
0
0
0
96
12 Lamberton, MinnesotaV
 BZ
96.83
0
4.69
4.69
56
2
0
3
3
0
0
0
0
0
96
UZ
96.43
10.71
0
0
40
2
6
0
0
0
0
0
0
0
48
13 Rosemount, MinnesotaV
 BZ
100
6.45
4.3
0
83
0
6
4
0
0
0
0
0
0
93
UZ
87.5
18.75
9.38
9.38
32
0
6
2
2
0
0
1
0
0
43
14 Brookings, South DakotaZ
UZ
94.44
1.85
3.7
3.7
46
3
1
2
2
0
0
0
0
0
54
15 South Shore, South DakotaZ
UZ
100
3.74
0
5.61
97
0
4
0
6
0
1
0
0
0
108
Total
98.66
3.96
3.47
2.19
1267
14
54
45
27
1
2
2
1
1
1414
Ecotypes (ET) represented are univoltine Z pheromone race (UZ), bivoltine Z pheromone race (BZ), univoltine E pheromone race (UE), or bivoltine E pheromone race (BE). Haplotypes defined using D = DdeI, H = HaeIII, S = Sau3AI, M = MspI, and w+ (wildtype) uncleaved.

Collections provided by:
R. Charles Mason 2001
U. David Handley 2002
X. Ron Seymour 2001
S. John Obermeyer 2001
V. Bruce Potter 2001 & 2002
Y. Wendel Roelofs 2001
T. Lawrence Buschman 2001
W. William Hutchison 2002
Z. Micheal Catangui 2001
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