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Table 1. Twenty-six variable nucleotide positions observed between 14 North American Ostrinia nubilalis mitochondrial cox1 and cox2 sequences. Random collection of sequences used to survey level and location of polymorphism among samples, such that PCR-RFLP assays could be developed for higher sample throughput. Positions with respect to the O. nubilalis mitochondrial genome sequence (GenBank AF442957), and – indicates sequence identical to the consensus sequence. RFLPs: D = DdeI, H = HaeIII, S = Sau3AI, and M = MspI are indicated. See Table 2 for location detail of collection site ID.
 
cox2
cox1
DNA sequence alignment position with respect to GenBank accession AF442957
Collection site ID / individual
1459
1529
2047
2062
2119
2161
2161
2169
2533
2531
2554
2829
2866
2928
2933
3050
3123
3429
3453
3462
3474
3477
3528
3544
3554
3555
Consensus
C
T
T
T
T
A
T
A
A
T
A
T
T
T
A
G
A
A
A
A
A
A
A
A
T
A
Complete Genome AF442957
A
C
C
A
T
G
T
G
G
G
C
G
Garden City, Kansas /♀17
G
G
T
T
T
G
T
T
Hubbard, Iowa /♂25
C
G
C
C
G
Brookings, South Dakota /♀06
A
T
G
Lamberton, Minnesota (BZ) /♂24
G
C
Mead, Nebraska /♂02
G
Kanawha, Iowa /♀10
T
Kanawha, Iowa /♀46
T
C
Lamberton, Minnesota (UZ) /♀26
C
Rosemount, Minnesota (UZ) /♀20
G
Oxford, Maine (UE) /♀06
G
Cape Elizabeth, Maine (BE) /♀47
C
Franklin Co., Indiana /♀04
T
G
T
Crawfordsville, Iowa /♀46
T
Cape Elizabeth, Maine (UE) /♀46
G
RFLP
 
D
 
 
 
 
 
 
 
 
H
 
 
 
M
S
 
 
 
 
 
 
 
 
 
 
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