Table 3. Effect of UV-B treatment on the infectivity of AcMNPV recombinants and a clone (AcMNPV-HPP) of the wild type virusa
.
UV-B Exposure Time
LC50 (OB/mm2)
2 h
4 h
Virus
S
NS
S
NS
Ratio (NS/S)
AcRFPCL14
8.6 (L1 = 5.6;
L2 = 15.6)
20.03 (L1 = 8.9;
L2 = 47.1)
–
–
2.33
pAcUW21-23GFP
1.11 (L1 = 0.82;
L2 = 1.46)
0.85 (L1 = 0.54;
L2 = 1.21)
0.68 (L1 = 0.55;
L2 = 0.84)
4.1 (L1 = 0.55;
L2 = 0.84)
8.04 (L1 = 4.1;
L2 = 16.0)
0.77 (2h)
6.03 (4h)
11.8 (4h)
Ac-HPP
0.34 (L1 = 0.23;
L2 = 0.48)
26.72 (L1 = 19.4;
L2 = 37.2)
–
–
78.6
aBioassays performed on 24 h old Trichoplusia ni larvae employing 75 larvae per dilution for infectivity studies.
S = shielded ; NS = not shielded; (–) not determined; (L1 = lower 95% C.I.; L2 = upper 95% C.I.). Values in the 4 h, NS column of pAc-23GFP are from two replications.