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Table 3. Effect of UV-B treatment on the infectivity of AcMNPV recombinants and a clone (AcMNPV-HPP) of the wild type virusa .

UV-B Exposure Time

LC50 (OB/mm2)

2 h

4 h

Virus

S

NS

S

NS

Ratio (NS/S)

AcRFPCL14

8.6 (L1 = 5.6;

L2 = 15.6)

20.03 (L1 = 8.9;

L2 = 47.1)

2.33

pAcUW21-23GFP

1.11 (L1 = 0.82;

L2 = 1.46)

0.85 (L1 = 0.54;

L2 = 1.21)

0.68 (L1 = 0.55;

L2 = 0.84)

4.1 (L1 = 0.55;

L2 = 0.84)

8.04 (L1 = 4.1;

L2 = 16.0)

0.77 (2h)

6.03 (4h)

11.8 (4h)

Ac-HPP

0.34 (L1 = 0.23;

L2 = 0.48)

26.72 (L1 = 19.4;

L2 = 37.2)

78.6

aBioassays performed on 24 h old Trichoplusia ni larvae employing 75 larvae per dilution for infectivity studies.

S = shielded ; NS = not shielded; (–) not determined; (L1 = lower 95% C.I.; L2 = upper 95% C.I.). Values in the 4 h, NS column of pAc-23GFP are from two replications.
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