 |
|
Table 1. In vitro and in vivo infectivity of AcMNPV recombinants and AcMNPV-HPPa a clone of the wild type virus.
|
Virus |
TCID50/ml ± SEM |
LC50 (OB/mm2) ± SEM |
AcRFPCL1 |
3.30 x 106 ± 0.001 |
63.00 ± 10.72 |
AcRFPCL2 |
1.15 x 108 ± 0.088 |
328.75 ± 49.3 |
AcRFPCL14 |
9.49 x 107 ± 0.001 |
20.10 ± 3.24 |
pAcUW21-23GFP |
1.15 x 106 ± 0.001 |
0.10 ± 0.02 |
AcMNPV-HPP |
1.15 x 108 ± 0.097 |
0.095 ± 0.02 |
|
|
aTN-CL1 cells were used for the generation of TCID50 values and 24 h old Trichoplusia ni larvae for conducting bioassays. Twenty-four wells per dilution were employed in 96 well plates for viral titrations and 75 larvae per dilution for infectivity studies.
|
 |