Figure 3. In vitro assays for promoter activity in the cloned 5'-untranscribed sequences of two Musca domestica hexamerin genes. S2 cells were transfected with the reporter plasmids and GFP expression was monitored by observation of the cultures in an inverted microscope under UV light. Transfections were conducted with 1) pGreenPelican-HexL: MdHexL1 promoter cloned into pGreenPelican; 2) pGreen-HexF: MdHexF1 promoter cloned into pGreenPelican; 3) pGreenPelican vector without insert; 4) pGreen-Actin-5C: Drosophila melanogaster actin-5C promoter cloned into pGreenPelican. A and B indicate different magnifications of the transfected cells.