Figure 6. Mean relative distribution and density of actin in Heliothis virescens hemocytes as measured by fluorescence. Hemocytes from control individuals were allowed to spread in PBS (ctl) or plasma from control (c/o) or larvae one day after parasitization (p/o), or were isolated from larvae one day after parasitization and allowed to spread in PBS (pp). Images were captured using (a) FITC and (b) rhodamine optics at or near the plane of adherence to the coverslip (z) and at z+2.5µm (cytoplasm). Error bars show standard errors of the mean. Different letters above bars indicate means are statistically different at z-focal distance at =0.05, different numbers above bars indicate significant difference at z+2.5µm.