Figure 2. Gel of amplified virus-specific DNA from Diaprepes root weevil. Lanes: 1. Ladder wide-range DNA marker, (16 fragments, 50-10,000 bp), 2. Blank, 3. Positive control purified iridescent virus-6 (IIV6) (amplified DNA fragment ~893 bp). 4. Blank. 5. Water control. 6. Adult weevil control injected with 4 µl of water. 7. Larvae weevil control injected with 4 µl of water. 8. Weevil egg control. 9. Adult weevil inoculated by puncturing with an insect pin dipped in purified virus. 10. Larvae inoculated by puncturing. 11. Adult weevil injected with 4 µl of homogenate made from an individual with a patent infection. 12. Larvae injected with 4 µl of homogenate virus inoculum. 13. Adult inoculated per os with purified IIV6 in 20 % sucrose (1 µl virus:10 µl sucrose solution). 14. Larvae inoculated per os. 15. Egg mass of 10-20 eggs from adult female inoculated by injection. 16. Egg mass of 10-20 eggs from adult female inoculated per os.