 |
|
Table 6 Comparison between EC50 values determined in the BII bioassay and Kd values in cell-free extracts of BII cells for a range of ecdysteroids and two bisacylhydrazines. See Lafont et al. (2002) for the structures of the unnumbered ecdysteroids.
|
| Ecdysteroid |
EC50 value*
| Kd value |
Kd/EC50 |
| ponasterone A (7) |
3.1 x 10-10M |
7.2 x 10-10M |
2.3 |
| ajugasterone C (8) |
3.0 x 10-8M |
1.3 x 10-9M |
0.04 |
| muristerone A (9) |
2.2 x 10-8M |
2.2 x 10-9M |
0.10 |
| 25-fluoro-20-hydroxyecdysone |
5.1 x 10-9M |
5.2 x 10-9M |
1.0 |
| polypodine B |
1.0 x 10-9M |
7.7 x 10-9M |
7.7 |
| 5-deoxykaladasterone |
5.2 x 10-10M |
1.0 x 10-8M |
19.2 |
| abutasterone |
1.4 x 10-7M |
1.1 x 10-8M |
0.08 |
| cyasterone |
1.2 x 10-8M |
1.7 x 10-8M |
1.4 |
| 20-hydroxyecdysone (6) |
7.5 x 10-9M |
2.5 x 10-8M |
3.3 |
| inokosterone |
1.1 x 10-7M |
3.5 x 10-8M |
0.17 |
| makisterone A |
1.3 x 10-8M |
6.4 x 10-8M |
4.9 |
| turkesterone (1) |
1.3 x 10-6M |
1.4 x 10-7M |
0.11 |
| RH5992 |
5.3 x 10-7M |
2.1 x 10-7M |
0.40 |
| ajugalactone |
1.6 x 10-7M |
3.1 x 10-7M |
0.19 |
| RH5849 |
1.8 x 10-6M |
1.5 x 10-7M |
0.08 |
| ecdysone |
1.1 x 10-6M |
1.7 x 10-6M |
1.55 |
| 2-deoxyecdysone |
5.0 x 10-5M |
3.8 x 10-6M |
0.08 |
| 22-epi-ecdysone |
5.3 x 10-6M |
6.7 x 10-6M |
1.26 |
| poststerone |
2.0 x 10-5M |
1.5 x 10-5M |
0.75 |
|
|
* values taken from Clément and Dinan (1993) or Dinan et al. (1999)
|
|