Figure 6. Immunoblots of Ms-golgin80 from tissue lysates and fusion proteins. A protein band of approximately 80 kilodaltons is present in lanes 3 and 6. There is a band of protein at 95 kilodaltons in lane 5. No protein bands are found in lane 1, 2, and 4. Lane 1: plain S2 cell; Lane 2: induced plain S2 cell; Lane 3: induced transfected S2 cell. Lane 4: non-induced transformed BL21 cell; Lane 5: induced transformed BL-21 cell; Lane 6: the larval CNS. BL-21 bacteria were transformed with 3-1-b1 plasmid (Uni-Zap), whereas the S2 cells were transfected with P-Met-3-1-b1 plasmid.